Target detection service - メーカー・企業と業務用製品 | イプロスものづくり

[Case Study] Off-Target Detection in Genome Editing Knock-In

We will introduce examples of off-target detection in knock-in during genome editing!

We would like to introduce a case study of our random integration analysis service using the RAIS method. For a knock-in mouse created using CRISPR/Cas, we attempted to detect the knock-in efficiency and off-target sites by combining the RAIS method with NGS. It was suggested that the donor DNA used for the knock-in was inserted not only into the intended chromosome 9 (chr9) but also into another chromosome. 【Random Integration Analysis Service using the RAIS Method Contents】 ■ Sanger Sequencing Plan ■ Next Generation Sequencing (NGS) Plan *For more details, please refer to the PDF document or feel free to contact us.